Article

Mutational scanning of TnpB reveals latent activity for genome editing

2025-02-12

Abstract excerpt

TnpB is a diverse family of RNA-guided endonucleases associated with prokaryotic transposons. Due to their small size and putative evolutionary relationship to CRISPR-Cas12, TnpB enzymes hold significant potential for genome editing. However, most TnpBs lack robust gene editing activity, and unbiased profiling of mutational effects on editing activity has not been explored. Here, we mapped comprehensive sequence-f...

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Literature Corpus work: ac701da9-d83f-53c2-8506-b9ecbac3bc9f
DOI: 10.1101/2025.02.11.637750

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