Bacteriophage genome-wide transposon mutagenesis

Abstract excerpt

Bacteriophages have genomes that span a wide size range, are densely packed with coding sequences, and frequently encode genes of unknown function. Classical forward genetics has defined essential genes for phage replication in a few model systems but remains laborious and non-scalable. Unbiased functional genomics approaches are therefore needed for phages, particularly for large lytic phages. Here, we develop a...

Topics

Open a Topic to create a Post that cites this publication.

• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• DNA Transposable Elements
• Gene Editing
• Immunity, Innate
• RNA, Bacterial
• RNA, Guide, CRISPR-Cas Systems
• Transposases

Identifiers and source

Literature Corpus work

94a1606e-3941-5dd0-a3aa-5f595467bd31

DOI

10.1101/2025.11.23.690004