Programmable DNA integration with New-to-Nature tools using Computational Protein Design

Abstract excerpt

Programmable integration of large DNA cargo (≥ 2 kb), without inducing double-strand breaks, remains challenging for genome editing technologies. Current approaches have limitations in programmability, depend on co-delivery of multiple components, require multiple enzymatic steps, or have variable on-target editing outcomes. Here, we address this challenge using de novo protein design to create highly active, new...

Topics

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• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Directed Molecular Evolution
• Endonucleases
• Gene Editing
• HEK293 Cells
• Mutation
• Protein Engineering

Identifiers and source

Literature Corpus work

f892dce7-582f-526b-af40-83b7fc1aacd2

DOI

10.64898/2026.05.29.728539