Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleases

Abstract excerpt

Transposon-encoded tnpB genes encode RNA-guided DNA nucleases that promote their own selfish spread through targeted DNA cleavage and homologous recombination 1–4 . This widespread gene family was repeatedly domesticated over evolutionary timescales, leading to the emergence of diverse CRISPR-associated nucleases including Cas9 and Cas12 5,6 . We set out to test the hypothesis that TnpB nucleases may have also...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• DNA Transposable Elements
• Gene Editing
• Immunity, Innate
• RNA, Bacterial
• RNA, Guide, CRISPR-Cas Systems
• Transposases

Identifiers and source

Literature Corpus work

19be19d3-dc63-5900-9b09-3caf9c1b37fe

DOI

10.1101/2023.11.30.569447