Article

Engineering transposon-associated TnpB-ωRNA system for efficient gene editing and disease treatment in mouse

2023-06-30

Abstract excerpt

Transposon-associated ribonucleoprotein TnpB is known to be the ancestry endonuclease of diverse Cas12 effector proteins from type-V CRISPR system. Given its small size (409 aa), it is of interest to examine whether engineered TnpB could be used for efficient mammalian genome editing. Here, we showed that the gene editing activity of native TnpB in mouse embryos was already higher than previously identified small-...

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Literature Corpus work: 705b1039-a46d-57c7-aba0-76e15aeebd12
DOI: 10.1101/2023.06.29.547137

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