Article
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases.
Science (New York, N.Y.) - 1 Oct 2021
Altae-Tran Han, Kannan Soumya, Demircioglu F Esra, Oshiro Rachel, Nety Suchita P, McKay Luke J, Dlakić Mensur, Inskeep William P, Makarova Kira S, Macrae Rhiannon K, Koonin Eugene V, Zhang Feng
Abstract excerpt
IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB and its interactions with any RNA remain uncharacterized. Using evolutionary analysis, RNA sequencing, and biochemical experiments, we reconstructed the evolution of CRISPR-Cas9 systems from IS200/IS605 transposons. We found that IscB...
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