Article
Comprehensive profiling of activity and specificity of RNA-guided transposons reveals opportunities to engineer improved variants.
Nucleic acids research - 23 Sept 2025
Park Seong Guk, Park Jung-Un, Dodero-Rojas Esteban, Bryant John A, Sankaranarayanan Geetha, Kellogg Elizabeth H
Abstract excerpt
Recently discovered CRISPR-associated transposons (CASTs) are natural RNA-guided DNA transposition systems capable of single-step genomic integration of large DNA cargo. Wild-type CASTs exhibit low integration activity in heterologous systems; therefore, engineering efforts are required to develop therapeutically relevant tools. Here we developed a high-throughput dual genetic screen capable of accurately...
Read the complete abstract on PubMedTopics
- DNA Transposable Elements
- CRISPR-Cas Systems
- RNA, Guide, CRISPR-Cas Systems
- Genetic Engineering
- Mutation
- Gene Editing