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Directed evolution expands CRISPR-Cas12a genome editing capacity

2025-03-26

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas12a enzymes are versatile RNA-guided genome-editing tools with applications encompassing viral diagnosis, agriculture and human therapeutics. However, their dependence on a 5’-TTTV-3’ protospacer-adjacent motif (PAM) next to DNA target sequences restricts Cas12a’s gene targeting capability to only ∼1% of a typical genome. To mitigate this constraint, we used a bacterial-based directed...

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Identifiers and source

Literature Corpus work
22a2c119-cb0f-55f1-9ff8-e3c1feaa56e1
DOI
10.1101/2025.03.26.645588
Open publication

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Directed evolution expands CRISPR-Cas12a genome editing capacityDOI 10.1101/2025.03.26.645588
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