Article

Structure and evolution-guided design of minimal RNA-guided nucleases

2025-12-08

Abstract excerpt

The design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome editing capabilities. However, generating diverse multi-domain proteins with robust enzymatic properties remains challenging. Here we use an artificial intelligence-driven strategy that couples structure-guided inverse protein folding with evolution-informed residue constraints to generate active, divergent v...

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Literature Corpus work: be7f5185-2dd3-554d-89cf-1dee1638d8f5
DOI: 10.64898/2025.12.08.692503

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