Article

Evaluating Computational Tools for CRISPR-Cas9 Design: A Case Study on the TP53 Gene

2026-08-31

Abstract excerpt

<title>Abstract</title> <p> While CRISPR-Cas9 has revolutionized genome editing entirely, successful gene knockouts still rely heavily on computational tools to select the right single-guide RNAs (gRNAs) that maximize on-target efficiency and minimize off-target risks. Because different <italic>in silico</italic> platforms use distinct scoring algorithms, their recommendations often vary. In this study, we com...

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Literature Corpus work: a1bbd174-bade-552d-9ca6-694d3776aef8
DOI: 10.21203/rs.3.rs-10762687/v1

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