Article

Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance

2020-02-20

Abstract excerpt

CRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens. However, recent work has raised concerns regarding the performance of CRISPR-Cas9 screens using TP53 wild-type human cells due to a p53-mediated DNA damage response (DDR) limiting the efficiency of generating viable edited cells. To directly assess the impact of cellular p53 status on CRISPR-Cas9 screen performance, we c...

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Literature Corpus work: f769f2a0-6367-50ba-90a4-6add86e62e5e
DOI: 10.1101/2020.02.20.957746

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