Article

KOnezumi-AID: A Software to Automate the Design of Multiplex Knockouts Using Target-AID

2024-11-03

Abstract excerpt

With the groundbreaking advancements in genome editing technologies, particularly CRISPR-Cas9, creating knockout mutants has become highly efficient. However, the CRISPR-Cas9 system introduces DNA double-strand breaks, which can increase the risk of chromosomal rear-rangements, presenting a major obstacle when attempting to knockout multiple genes simultaneously. Base editing systems like Target-AID provide a safe...

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Literature Corpus work: 23856f2b-5a78-54cd-9d17-123d9e9b7db9
DOI: 10.1101/2024.10.29.620976

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