Article
Cas9 activates the p53 pathway and selects for p53-inactivating mutations.
Nature genetics - 1 Jul 2020
Enache Oana M, Rendo Veronica, Abdusamad Mai, Lam Daniel, Davison Desiree, Pal Sangita, Currimjee Naomi, Hess Julian, Pantel Sasha, Nag Anwesha, Thorner Aaron R, Doench John G, Vazquez Francisca, Beroukhim Rameen, Golub Todd R, Ben-David Uri
Abstract excerpt
Cas9 is commonly introduced into cell lines to enable CRISPR-Cas9-mediated genome editing. Here, we studied the genetic and transcriptional consequences of Cas9 expression itself. Gene expression profiling of 165 pairs of human cancer cell lines and their Cas9-expressing derivatives revealed upregulation of the p53 pathway upon introduction of Cas9, specifically in wild-type TP53 (TP53-WT) cell lines. This was...
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