Article

Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

2024-04-11

Abstract excerpt

<h4>SUMMARY</h4> Human DNA licensing initiates the process of replication fork assembly. Specifically, this reaction leads to the loading of hMCM2-7 on DNA, which represents the core of the replicative helicase that unwinds DNA during S-phase. Here, we report the biochemical reconstitution of human DNA licensing using purified proteins, the structural and functional analysis of the process and reveal the impact o...

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Literature Corpus work: 96189190-758e-5988-9b0a-d5ee5a98aa3d
DOI: 10.1101/2024.04.11.589023

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