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MCM Double Hexamer Loading Visualised with Human Proteins

2024-04-10

Abstract excerpt

Eukaryotic DNA replication begins with the loading of the MCM replicative DNA helicase as a head-to-head double hexamer (DH) at origins of DNA replication 1–3 . Our current understanding of how DH is assembled by the Origin Recognition Complex (ORC), CDC6 and CDT1 comes mostly from budding yeast. Here we characterise human DH (hDH) loading using biochemical reconstitution and cryo-electron microscopy with purifie...

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Identifiers and source

Literature Corpus work
a80f0054-eb1f-5f35-921f-44d5ac749a45
DOI
10.1101/2024.04.10.588848
Open publication

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MCM Double Hexamer Loading Visualised with Human ProteinsDOI 10.1101/2024.04.10.588848
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