Article

Development of compact transcriptional effectors using high-throughput measurements in diverse contexts

2023-05-14

Abstract excerpt

Human nuclear proteins contain >1000 transcriptional effector domains that can activate or repress transcription of target genes. We lack a systematic understanding of which effector domains regulate transcription robustly across genomic, cell-type, and DNA-binding domain (DBD) contexts. Here, we developed dCas9-mediated high-throughput recruitment (HT-recruit), a pooled screening method for quantifying effector f...

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Literature Corpus work: 9318cccf-6a2a-5bc4-a973-8a72bb05b7fc
DOI: 10.1101/2023.05.12.540558

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