Article
Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells.
Molecular cell - 20 Apr 2017
Xie Shiqi, Duan Jialei, Li Boxun, Zhou Pei, Hon Gary C
Abstract excerpt
The study of enhancers has been hampered by the scarcity of methods to systematically quantify their endogenous activity. We develop Mosaic-seq to systematically perturb enhancers and measure their endogenous activities at single-cell resolution. Mosaic-seq uses a CRISPR barcoding system to jointly measure a cell's transcriptome and its sgRNA modulators, thus quantifying the effects of dCas9-KRAB-mediated...
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