Article
High-Throughput Discovery and Characterization of Human Transcriptional Effectors.
Cell - 23 Dec 2020
Tycko Josh, DelRosso Nicole, Hess Gaelen T, Aradhana, Banerjee Abhimanyu, Mukund Aditya, Van Mike V, Ego Braeden K, Yao David, Spees Kaitlyn, Suzuki Peter, Marinov Georgi K, Kundaje Anshul, Bassik Michael C, Bintu Lacramioara
Abstract excerpt
Thousands of proteins localize to the nucleus; however, it remains unclear which contain transcriptional effectors. Here, we develop HT-recruit, a pooled assay where protein libraries are recruited to a reporter, and their transcriptional effects are measured by sequencing. Using this approach, we measure gene silencing and activation for thousands of domains. We find a relationship between repressor function and...
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