Back to search

Article

High-throughput development and characterization of new functional nanobodies for gene regulation and epigenetic control in human cells

2024-11-03

Abstract excerpt

Controlling gene expression and chromatin state via the recruitment of transcriptional effector proteins to specific genetic loci has advanced the potential of mammalian synthetic biology, but is still hindered by the challenge of delivering large chromatin regulators. Here, we develop a new method for generating small nanobodies against human chromatin regulators that can repress or activate gene expression. We s...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
889036fd-52ae-5aed-a0be-005b698566da
DOI
10.1101/2024.11.01.621523
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
High-throughput development and characterization of new functional nanobodies for gene regulation and epigenetic control in human cellsDOI 10.1101/2024.11.01.621523
Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human ProteinsSemantic proximity
High-throughput functional characterization of combinations of transcriptional activators and repressorsSemantic proximity
Large-scale mapping and systematic mutagenesis of human transcriptional effector domainsSemantic proximity
Evolution of nanobodies specific for BCL11ASemantic proximity
Humanized Caffeine-Inducible Systems for Controlling Cellular FunctionsSemantic proximity
FLASH assembly of TALENs for high-throughput genome editing.Semantic proximity
High-throughput discovery and characterization of human transcriptional effectorsSemantic proximity
Transposon-Display of AI-designed binders enables manipulation of the proteome in human cellsSemantic proximity
A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbationSemantic proximity
High-Throughput Discovery and Characterization of Human Transcriptional Effectors.Semantic proximity
Discovery of hypercompact epigenetic modulators for persistent CRISPR-mediated gene activationSemantic proximity
High-throughput discovery of regulatory effector domains in human RNA-binding proteinsSemantic proximity
Select a neighboring publication to make it the new centre.