Article

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cells

2025-05-15

Abstract excerpt

<h4>ABSTRACT</h4> Transposon-Display is a highly scalable screening method that links proteins to their encoding DNA during expression in E. coli via a mutant transposase. Leveraging this system, we identified AI-designed binders targeting four intracellular human proteins, saturation-mutagenized top candidates, and charge-balanced variants to improve compatibility with the intracellular environment while preser...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 14ddd52b-e56a-520e-9d6a-c8bdafdda84b
DOI: 10.1101/2025.05.11.653342

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cellsDOI 10.1101/2025.05.11.653342Read

Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human ProteinsSemantic proximity Read

Rational design of synthetic proteins using a genome-scale CRISPR screenSemantic proximity Read

Engineered Peptide Barcodes for In-Depth Analyses of Binding Protein EnsemblesSemantic proximity Read

A scalable strategy for high-throughput GFP tagging of endogenous human proteinsSemantic proximity Read

AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modificationsSemantic proximity Read

A strategy for genome-wide seamless tagging of human protein-coding genesSemantic proximity Read

Gene-sized DNA insertion at genomic safe harbors in human cells using a site-directed transposaseSemantic proximity Read

Large-scale discovery of recombinases for integrating DNA into the human genomeSemantic proximity Read

Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody librariesSemantic proximity Read

Harnessing fusion of genome-edited human stem cells to rapidly screen for novel protein functions <i>in vivo</i>Semantic proximity Read

Evolution of nanobodies specific for BCL11ASemantic proximity Read

Baculovirus-vectored precision delivery of large DNA cargoes in human genomesSemantic proximity Read