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Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins

5 Oct 2015

Abstract excerpt

We developed and optimized a high-throughput project workflow to generate renewable recombinant antibodies to human proteins involved in epigenetic signalling. Three different strategies to produce phage display compatible protein antigens in bacterial systems were compared, and we found that in vivo biotinylation through the use of an Avi tag was the most productive method. Phage display selections were...

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PMID: 26437229
DOI: 10.1371/journal.pone.0139695

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