Article

High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

2020-12-03

Abstract excerpt

<h4>ABSTRACT</h4> Precise and efficient insertion of large DNA fragments into somatic cells using gene editing technologies to label or modify endogenous proteins remains challenging. Non-specific insertions/deletions (INDELs) resulting from the non-homologous end joining pathway make the process error-prone. Further, the insert is not readily removable. Here, we describe a method called CRISPR -mediated inserti...

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Literature Corpus work: 7d18185f-2ad0-5805-bb31-3a23c8f56768
DOI: 10.1101/2020.12.03.409417

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