Article

Comprehensive analysis of end-modified long dsDNA donors in CRISPR-mediated endogenous tagging

2024-06-29

Abstract excerpt

CRISPR-mediated endogenous tagging is a powerful gene editing technique for studying protein dynamics and function in their native cellular environment. While the use of 5’ modified DNA donors has emerged as a promising strategy to improve the typically low efficiency of knock-in gene editing, the underlying mechanisms remain poorly understood. In this study, we conducted a comprehensive analysis of end-modified l...

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Literature Corpus work: 35c9bfed-5ba7-5e4c-ba75-9a4031b934d1
DOI: 10.1101/2024.06.28.601124

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