Article

A strategy for genome-wide seamless tagging of human protein-coding genes

2025-03-11

Abstract excerpt

Comprehensive and systematic proteome-wide experiments require financial and technical resources unavailable to most researchers. Here we describe a scalable CRISPR/Cas9-non-homologous end joining (NHEJ) based method for P ooled R ecombinant I ntegration of S eamless M arkers (PRISM) into protein coding genes. We created two gRNA libraries for 5’- and 3’-tagging of 18,804 human protein-coding genes. Selection...

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Literature Corpus work: 40d2273f-538e-5737-9c1d-24dadd8675cb
DOI: 10.1101/2025.03.04.641506

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