Article

Programmable multi-kilobase RNA editing using CRISPR-mediated trans-splicing

2023-08-18

Abstract excerpt

Current gene editing approaches in eukaryotic cells are limited to single base edits or small DNA insertions and deletions, and remain encumbered by unintended permanent effects and significant challenges in the delivery of large DNA cargo. Here we describe Splice Editing, a generalizable platform to correct gene transcripts in situ by programmable insertion or replacement of large RNA segments. By combining CRIS...

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Literature Corpus work: 6c08d2f6-c74f-5ee3-9658-563876f3fe14
DOI: 10.1101/2023.08.18.553620

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