Article

<i>Easi</i> -CRISPR: Efficient germline modification with long ssDNA donors

2016-08-17

Abstract excerpt

CRISPR/Cas9 technology efficiently produces short insertions or deletions ( indels ) and can insert short exogenous sequences at Cas9 cut sites. However, targeting long inserts is still a major technical challenge. To overcome this challenge, we developed Easi -CRISPR ( E fficient a dditions with s sDNA i nserts-CRISPR), a method that uses long, in vitro -synthesized, single-stranded DNAs with 50-100 base h...

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Literature Corpus work: 331f314e-ced7-5934-8196-b4d7ff2937df
DOI: 10.1101/069963

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