Article

An <i>in situ</i> cut-and-paste genome editing platform mediated by CRISPR/Cas9 or Cas12a

2022-03-31

Abstract excerpt

<h4>ABSTRACT</h4> Recombinant DNA technology mediated by restriction enzymes and ligases allows in vitro manipulation of a DNA segment isolated from the genome. Short overhangs generated by restriction enzymes facilitate efficient pasting together a DNA sequence and a vector. We adopted this recombinant DNA strategy to develop an in vivo recombinant-genome genome editing approach. Using the programmable endonuc...

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Literature Corpus work: 8e224d99-6a2f-51c0-b79e-ecc817248258
DOI: 10.1101/2022.03.30.486486

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