Article

<i>In Vivo</i> Blunt-End Cloning Through CRISPR/Cas9-Facilitated Non-Homologous End-Joining

2015-05-21

Abstract excerpt

The ability to precisely modify the genome in a site-specific manner is extremely useful. The CRISPR/Cas9 system facilitates precise modifications by generating RNA-guided double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called knock-in blunt ligation for exploiting thi...

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Literature Corpus work: f44fdb2e-3a4d-5289-84d3-56b9240c4986
DOI: 10.1101/019570

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