Article

Rational and computation-assisted engineering of a compact and efficient StaCas9 genome editor

2026-07-21

Abstract excerpt

Type II-A CRISPR-Cas9 nucleases are widely used for genome editing, yet their functional diversity and therapeutic potential remain incompletely explored. In this study, we systematically analyzed natural variation in PAM recognition among SaCas9 orthologs and identified StaCas9 as a compact and efficient nuclease recognizing an NNG PAM. Structural and sequence analyses revealed that S983 within the PAM-interactin...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6c13526d-e4ee-5935-9e0a-56b8aaf82f04
DOI: 10.64898/2026.07.21.739771

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Rational and computation-assisted engineering of a compact and efficient StaCas9 genome editorDOI 10.64898/2026.07.21.739771Read

A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing.Semantic proximity Read

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Semantic proximity Read

Engineered CRISPR-Cas9 nucleases with altered PAM specificities.Semantic proximity Read

Closely related type II-C Cas9 orthologs recognize diverse PAMsSemantic proximity Read

In-depth assessment of the PAM compatibility and editing activities of Cas9 variants.Semantic proximity Read

Diverse noncanonical PAMs recognized by SpCas9 in human cellsSemantic proximity Read

Divergent PAM Specificity of a Highly-Similar SpCas9 OrthologSemantic proximity Read

Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9Semantic proximity Read

Genome editing mediated by SpCas9 variants with broad non-canonical PAM compatibility in plants.Semantic proximity Read

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor.Semantic proximity Read

GenomePAM directs PAM characterization and engineering of CRISPR-Cas nucleases using mammalian genome repeatsSemantic proximity Read

CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomesSemantic proximity Read