Article

Diverse noncanonical PAMs recognized by SpCas9 in human cells

2019-06-14

Abstract excerpt

The CRISPR/Cas9 system derived from Streptococcus pyogenes (SpCas9) provides unprecedented genome editing capabilities, but the potential for off-target mutations limits its application. In addition to NGG protospacer adjacent motif (PAM), off-target mutations are also associated with noncanonical PAMs, which have not yet been systematically evaluated. Here, we developed a highly sensitive approach that allows sy...

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Literature Corpus work: be4624fc-99a5-5789-8c8c-0d13551fd06f
DOI: 10.1101/671503

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