Article
In-depth assessment of the PAM compatibility and editing activities of Cas9 variants.
Nucleic acids research - 7 Sept 2021
Zhang Weiwei, Yin Jianhang, Zhang-Ding Zhengrong, Xin Changchang, Liu Mengzhu, Wang Yuhong, Ai Chen, Hu Jiazhi
Abstract excerpt
A series of Cas9 variants have been developed to improve the editing fidelity or targeting range of CRISPR-Cas9. Here, we employ a high-throughput sequencing approach primer-extension-mediated sequencing to analyze the editing efficiency, specificity and protospacer adjacent motif (PAM) compatibility of a dozen of SpCas9 variants at multiple target sites in depth, and our findings validate the high fidelity or...
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