Article
Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature - 23 Jul 2015
Kleinstiver Benjamin P, Prew Michelle S, Tsai Shengdar Q, Topkar Ved V, Nguyen Nhu T, Zheng Zongli, Gonzales Andrew P W, Li Zhuyun, Peterson Randall T, Yeh Jing-Ruey Joanna, Aryee Martin J, Joung J Keith
Abstract excerpt
Although CRISPR-Cas9 nucleases are widely used for genome editing, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM). As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome-editing applications. The ability to engineer Cas9 derivatives with purposefully altered...
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