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Article

Genome editing mediated by SpCas9 variants with broad non-canonical PAM compatibility in plants.

Molecular plant - 1 Feb 2021

Li Juan, Xu Rongfang, Qin Ruiying, Liu Xiaoshuang, Kong Fanna, Wei Pengcheng

Abstract excerpt

Streptococcus pyogenes Cas9 (SpCas9) is the most widely used genome editing tool in plants. The editing induced by SpCas9 strictly requires a canonical NGG protospacer-adjacent motif (PAM), significantly limiting its scope of application. Recently, five SpCas9 variants, SpCas9-NRRH, SpCas9-NRCH, SpCas9-NRTH, SpG, and SpRY, were developed to recognize non-canonical PAMs in human cells. In this study, these...

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Share to CRISPR-Associated Protein 9
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 33383203
DOI: 10.1016/j.molp.2020.12.017

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