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Article

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.

Science (New York, N.Y.) - 17 Apr 2020

Walton Russell T, Christie Kathleen A, Whittaker Madelynn N, Kleinstiver Benjamin P

Abstract excerpt

Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove this constraint, we engineered variants of Streptococcus pyogenes Cas9 (SpCas9) to eliminate the NGG PAM requirement. We developed a variant named SpG that is capable of targeting an expanded set of NGN PAMs, and we further optimized this...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32217751
DOI: 10.1126/science.aba8853
PMCID: PMC7297043

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