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Article

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor.

Nature communications - 25 Nov 2021

Wang Jian, Teng Yuxi, Zhang Ruihua, Wu Yifei, Lou Lei, Zou Yusong, Li Michelle, Xie Zhong-Ru, Yan Yajun

Abstract excerpt

The RNA-guided CRISPR-associated Cas9 proteins have been widely applied in programmable genome recombination, base editing or gene regulation in both prokaryotes and eukaryotes. SpCas9 from Streptococcus pyogenes is the most extensively engineered Cas9 with robust and manifold functionalities. However, one inherent limitation of SpCas9 is its stringent 5'-NGG-3' PAM requirement that significantly restricts its...

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Identifiers

PMID: 34824292
DOI: 10.1038/s41467-021-27290-9
PMCID: PMC8617050

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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