GenomePAM directs PAM characterization and engineering of CRISPR-Cas nucleases using mammalian genome repeats

Abstract excerpt

<title>Abstract</title> <p>Characterizing the protospacer adjacent motif (PAM) requirements of different Cas enzymes is a bottleneck in the discovery of Cas proteins and their engineered variants in mammalian cell contexts. To overcome this challenge and to enable more scalable characterization of PAM preferences, we develop a method named GenomePAM that allows for direct PAM characterization in mammalian cells....

Topics

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• CRISPR and Genetic Engineering
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Gene Editing
• Gene Targeting
• HEK293 Cells
• Mutagenesis
• Protein Domains

Identifiers and source

Literature Corpus work

d78bb1e7-fe6e-56c2-843d-857984d849f4

DOI

10.21203/rs.3.rs-4552906/v1