Article
A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing.
Science advances - 10 Feb 2023
Wang Yao, Qi Tao, Liu Jingtong, Yang Yuan, Wang Ziwen, Wang Ying, Wang Tianyi, Li Miaomiao, Li Mingqing, Lu Daru, Chang Alex Chia Yu, Yang Li, Gao Song, Wang Yongming, Lan Feng
Abstract excerpt
The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide mutations cannot be discriminated from wild-type alleles by current CRISPR-Cas systems. Here, we functionally screened six Cas12j nucleases and determined Cas12j-8 as an ideal genome editor with a hypercompact size. Cas12j-8 displayed comparable...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.