Article

Closely related type II-C Cas9 orthologs recognize diverse PAMs

2022-02-21

Abstract excerpt

Autosomal dominant diseases can be treated by allele-specific disruption of mutant alleles. If the missense mutation generates a novel protospacer-adjacent motif (PAM), CRISPR-Cas9 can distinguish mutant alleles from wild-type alleles by a PAM-specific approach. Therefore, it is crucial to develop a CRISPR toolbox capable of recognizing multiple PAMs. Here, by using a GFP-activation assay, we tested the activities...

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Identifiers and source

Literature Corpus work: fa165694-e467-5819-996d-6f59eba2440b
DOI: 10.1101/2022.02.21.481263

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