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Article

Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9

2024-11-25

Abstract excerpt

<title>Abstract</title> <p><italic>Staphylococcus aureus</italic> Cas9 (SaCas9), which is smaller than the widely-used <italic>Streptococcus pyogenes</italic> Cas9 (SpCas9), has been harnessed for gene therapy using an adeno-associated virus vector. However, SaCas9 requires an NNGRRT (where N is any nucleotide and R is A or G) protospacer adjacent motif (PAM) for target DNA recognition, thereby restricting the ta...

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Identifiers and source

Literature Corpus work
f82eaadb-c1ac-56e8-be60-751e905263e8
DOI
10.21203/rs.3.rs-4619764/v1
Open publication

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Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9DOI 10.21203/rs.3.rs-4619764/v1
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