Article

Divergent PAM Specificity of a Highly-Similar SpCas9 Ortholog

2018-02-02

Abstract excerpt

RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. The range of sequences that CRISPR endonucleases can recognize, however, is constrained by the need for a specific protospacer adjacent motif (PAM) flanking the target site. In this study, we demonstrate the natural PAM plasticity of a highly-similar, yet pre...

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Literature Corpus work: 9eeee6bc-8fb3-5a8e-ab5d-d02c5e8d4671
DOI: 10.1101/258939

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