Article

Cytosine base editing systems with minimized off-target effect and molecular size

2021-04-28

Abstract excerpt

<title>Abstract</title> <p>Structure-based rational engineering of the cytosine base editing system Target-AID was performed to minimize its off-target effect and molecular size. By intensive and careful truncation, DNA-binding domain of its deaminase PmCDA1 was eliminated and additional mutations were introduced to restore enzyme function. The resulting tCDA1EQ was effective in N-terminal fusion (AID-2S) or inla...

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Literature Corpus work: 52db6cc3-22fa-5eb2-a245-d24fb63eaa19
DOI: 10.21203/rs.3.rs-414230/v1

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