Article
Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain.
Nature cell biology - 1 Jun 2020
Zhang Xiaohui, Chen Liang, Zhu Biyun, Wang Liren, Chen Caiyu, Hong Mengjia, Huang Yifan, Li Huiying, Han Honghui, Cai Bailian, Yu Weishi, Yin Shuming, Yang Lei, Yang Zuozhen, Liu Meizhen, Zhang Ying, Mao Zhiyong, Wu Yuxuan, Liu Mingyao, Li Dali
Abstract excerpt
Cytidine base editors are powerful genetic tools that catalyse cytidine to thymidine conversion at specific genomic loci, and further improvement of the editing range and efficiency is critical for their broader applications. Through insertion of a non-sequence-specific single-stranded DNA-binding domain from Rad51 protein between Cas9 nickase and the deaminases, serial hyper cytidine base editors were generated...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.