Towards optimizing diversifying base editors for high-throughput studies of single- nucleotide variants

Abstract excerpt

Determining the phenotypic effects of single nucleotide variants is critical for understanding the genome and interpreting clinical sequencing results. Base editors, including diversifying base editors that create C>N mutations, are potent tools for installing point mutations in mammalian genomes and studying their effect on cellular function. Numerous base editor options are available for such studies, but little...

Topics

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• Adenosine Deaminase
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Cytosine
• Escherichia coli
• Gene Editing
• Mutation
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

1ef99eb9-1b19-5c37-9129-f8c9019e9cb3

DOI

10.1101/2024.11.18.621003