Article
Engineering a precise adenine base editor with minimal bystander editing.
Nature chemical biology - 1 Jan 2023
Chen Liang, Zhang Shun, Xue Niannian, Hong Mengjia, Zhang Xiaohui, Zhang Dan, Yang Jing, Bai Sijia, Huang Yifan, Meng Haowei, Wu Hao, Luan Changming, Zhu Biyun, Ru Gaomeng, Gao Hongyi, Zhong Liping, Liu Meizhen, Liu Mingyao, Cheng Yiyun, Yi Chengqi, Wang Liren, Zhao Yongxiang, Song Gaojie, Li Dali
Abstract excerpt
Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but their bystander mutations and off-target editing effects raise safety concerns. Through structure-guided engineering, we found ABE8e with an N108Q mutation reduced both adenine and cytosine bystander editing, and introduction of an additional L145T mutation (ABE9), further refined the editing window to 1-2 nucleotides with...
Topics
- Animals
- Mice
- Rats
- Gene Editing
- Adenine
- Mutation
- Cytosine
- CRISPR-Cas Systems
- RNA, Guide, CRISPR-Cas Systems