Article

Protocol for examining and eliminating base editor-induced genome-wide and transcriptome-wide off-target mutations

2021-05-12

Abstract excerpt

<title>Abstract</title> <p>Fusion of CRISPR-Cas9 with cytidine deaminases leads to base editors (BEs) for programmable C-to-T editing, which holds potentials in clinical applications but suffers from off-target (OT) mutations. Here, we applied a cleavable deoxycytidine deaminase inhibitor (dCDI) domain to construct a transformer BE (tBE) system that induces efficient editing with only background levels of genome-...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 294cb0b7-141c-58fa-ab11-1aa61f815760
DOI: 10.21203/rs.3.pex-1433/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Protocol for examining and eliminating base editor-induced genome-wide and transcriptome-wide off-target mutationsDOI 10.21203/rs.3.pex-1433/v1Read

Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations.Semantic proximity Read

Improved cytosine base editors generated from TadA variants.Semantic proximity Read

Progression and application of CRISPR-Cas genomic editors.Semantic proximity Read

Single‐nucleotide editing: From principle, optimization to applicationSemantic proximity Read

Towards optimizing diversifying base editors for high-throughput studies of single- nucleotide variantsSemantic proximity Read

TadA reprogramming to generate potent miniature base editors with high precision.Semantic proximity Read

Genome Editing With Targeted DeaminasesSemantic proximity Read

High-fidelity base editor with no detectable genome-wide off-target effectsSemantic proximity Read

Breaking genetic shackles: The advance of base editing in genetic disorder treatmentSemantic proximity Read

Design and application of the transformer base editor in mammalian cells and mice.Semantic proximity Read

Enhanced C-To-T and A-To-G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED.Semantic proximity Read

Next-generation cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activitySemantic proximity Read