Article

High-fidelity base editor with no detectable genome-wide off-target effects

2020-02-09

Abstract excerpt

Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we foun...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f3b22fc4-9b3b-527c-9775-7bedcfa02a39
DOI: 10.1101/2020.02.07.939074

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

High-fidelity base editor with no detectable genome-wide off-target effectsDOI 10.1101/2020.02.07.939074Read

Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activitySemantic proximity Read

Next-generation cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activitySemantic proximity Read

A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.Semantic proximity Read

Therapeutic adenine base editor with minimized off-target effects.Semantic proximity Read

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity.Semantic proximity Read

Single‐nucleotide editing: From principle, optimization to applicationSemantic proximity Read

Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA.Semantic proximity Read

Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.Semantic proximity Read

Engineering of cytosine base editors with DNA damage minimization and editing scope diversificationSemantic proximity Read

CRISPR adenine and cytosine base editors with reduced RNA off-target activitiesSemantic proximity Read

Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing.Semantic proximity Read

Advances in base editing: A focus on base transversions.Semantic proximity Read