Article

Engineering precise adenine base editor with infinitesimal rates of bystander mutations and off-target editing

2022-08-13

Abstract excerpt

Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but their bystander mutations and off-target editing effects raise the concerns of safety issues. Through structure-guided engineering, we found ABE8e with an N108Q mutation reduced both adenine and cytosine bystander editing, and introduction of an additional L145T mutation (ABE9), further refined the editing window to 1-2nt with...

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Literature Corpus work: 86a56a81-9931-5abf-9c35-987c0d6ae47b
DOI: 10.1101/2022.08.12.503700

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