Article

Reprogramming the antigen specificity of B cells using genomeediting technologies

2018-10-29

Abstract excerpt

We have developed a method to introduce novel paratopes into the human antibody repertoire by modifying the immunoglobulin genes of mature B cells directly using genome editing technologies. We used CRISPR-Cas9 in a homology directed repair strategy, to replace the heavy chain (HC) variable region in B cell lines with that from an HIV broadly neutralizing antibody, PG9. Our strategy is designed to function in cell...

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Literature Corpus work: 41fd774c-5367-53f6-bbe4-3da33c29e33c
DOI: 10.1101/455402

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