Article

<i>In vivo</i> affinity maturation of murine B cells reprogrammed to express human antibodies

2023-10-23

Abstract excerpt

CRISPR-edited murine B cells engineered to express human antibody variable chains proliferate, class switch, and secrete these antibodies in vaccinated mice. However, current strategies disrupt the heavy-chain locus, resulting in inefficient somatic hypermutation without functional affinity maturation. Here we show that recombined murine heavy- and kappa-variable genes can be directly and simultaneously overwritte...

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Literature Corpus work: b9c5af35-d745-52f3-aee5-55c9985e6bfc
DOI: 10.1101/2023.10.20.563154

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