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Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system

9 Mar 2016

Abstract excerpt

Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes. By delivering Cas9 and guide-RNA (gRNA) with retro- or lenti-virus to IgM(+) mouse B cells and hybridomas, we induce...

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PMID: 26956543
DOI: 10.1038/ncomms10934

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